Malt Dextrose Agar [MDA] + Peptone Mix 200g
This mixture is a favorite of mine and I'm sure you will find it works wonders for your cultures in the lab. This mix is specifically built to ensure your culture has access to a wide variety of nutrients in the form of malt extract, soy peptone, and dextrose all held together with a helping of agar. Available with no coloring added or in MycoPals 'True Blue' for added contrast. Adding color to your culture medium makes your plates visually pleasing and it can make things easier to see. Adding color will not negatively affect your cultures.
Directions for use
Use 25g per 500ml water | Good for 20 plates.
This 200g bag is able to pour 8 sleeves [160 plates] in its entirety.
The calculation above is based on 25ml poured per dish.
Powder preparation ratio is 0.05g powder to 1ml of water.
1ml of water = 1gram of water.
Prepare to pour
Place desired weight of powder into required amount of water.
Heat to boiling, dissolve mix completely.
Sterilize by autoclaving at 15psi (121c) for 15 minutes.
Allow to cool to 113 - 122F.
Mix well by shaking. Slight sediment at this stage, if any, dissolves completely in autoclave.
Pour onto sterile plates with aseptic technique.
Agar begins to gel up around 104F.
Pouring your plates in a sweet spot where the agar has not yet created a problem by gelling up on you, a temperature just above 104 and just below 117, will help you eliminate or minimize the formation of condensation on your plates.
Soy Based Peptone